Research interest
The kidney plays an essential role in regulating blood pressure and water balance by amending the urinary excretion of water and electrolytes, in conditions of deprivation or excess. Dysregulation of these processes can severely affect blood pressure, alter systemic fluid balance, and impair the maintenance of serum electrolytes within normal range.
The aim of our research is to understand the molecular mechanisms regulating channels and transporters that are involved in these processes, hereby particularly focusing on the molecular regulation of the thiazide-sensitive sodium chloride transporter NCC and the water channel Aquaporin-2 in kidney, during physiological and pathological conditions.
Methods and techniques
- Western blotting
- immunohistochemistry, immunocytochemistry, immunofluorescence
- RT-PCR and quantitative PCR
- immunoprecipitation, cell-surface biotinylation
- ELISA, signal transduction assays
- cell culture, stable and transient transfection
- transgenic mice models, metabolic balance studies in mice
- ex vivo murine and human tubule suspensions.
- Analyses of human kidney tissue
Funding
Independent Research Fund Denmark
A.P. Møller Foundation
Helen and Ejnar Bjørnows Foundation
Agnes og Poul Friis Foundation
Members of the research group
Gitte Kitlen - Lab technician
Anette Rasmussen – Lab technician
Mie Rytz Hansen - Lab technician
Nete Kronborg Vandel - Lab technician
Lene B. Andersen – Lab technician
Anja Toft - Lab technician intern
Information for students
Motivated students at any level who would like to learn more about our research and available positions are welcome to contact Marleen at kortenoeven@health.sdu.dk.
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