Line Lauritsen, Maria Szomek, Mick Hornum, Peter Reinholdt, Jacob Kongsted, Poul Nielsen, Jonathan R. Brewer & Daniel Wüstner

Ratiometric fuorescence nanoscopy and lifetime imaging of novel Nile Red analogs for analysis of membrane packing in living cells

Subcellular membranes have complex lipid and protein compositions, which give rise to organellespecifc membrane packing, fuidity, and permeability. Due to its exquisite solvent sensitivity, the lipophilic fuorescence dye Nile Red has been used extensively to study membrane packing and polarity.