Steno Diabetes Center Odense aims to become the driving force of diabetes research in the Region of Southern Denmark. We are always interested in collaborating in both national and international research project of relevance for people at risk of developing type 2 diabetes or patients with type 2 diabetes.
We have experience with a vast range of clinical and molecular methods and techniques, of which some are carried out in collaboration with other research units and departments.
Collaborations
Hyperinsulinaemic- euglycaemic and hyperglycaemic clamp techniques combined with tracer technology and indirect calorimetry (Oxycon Pro) for the evaluation of insulin action and insulin secretion in vivo and for the estimation of:
- Whole-body glucose disposal (insulin sensitivity)
- Endogenous (hepatic) glucose production
- Glucose and lipid oxidation
- Non-oxidative glucose metabolism (glycogen storage) and glycolytic flux
Oral glucose tolerance test (OGTT) and frequently-sampled intravenous glucose tolerance test (FSIGT/IVGTT) for the estimation of:
- Insulin secretion
- Insulin sensitivity
- Glucose effectiveness
Skeletal muscle and subcutaneous fat biopsies a. m. Bergström for:
- Establishment cultured skeletal muscle cells and adipocytes
- Isolation of mitochondria for mesures of respiration
- Electron microscopy for morphometric studies of of e.g. glycogen, lipids and mitochondria
- Studies of mRNA expression including transcriptional profiling
- Studies of abundance, activity and phosphorylation of enzymes/ proteins including Western blotting and proteomics