Research interest
Our research aim is to gain an understanding of the post-translational regulation of epithelial sodium transport under physiological and pathophysiological conditions. The experimental approaches are a combination of electrophysiological, imaging and molecular techniques carried out primarily on human tissue samples and cell cultures.
Methods and Techniques
- Immunohistochemistry (light-, laser scanning confocal-, multi-photon microscopy),
- Cloning
- Cell culture (Transient and stable expression systems, knock-down, CRISPR)
- Western blotting
- Quantitative PCR
- Studies on human kidney tissue
- Various transgenic and cell-specific knockout models
- Metabolic studies of renal function in animals
- Developmental changes in renal morphology and function
- Recombinant protein expression and purification
- Antibody development and characterization
- Electrophysiology: Patch Clamp (Whole-cell patch configuration, single channel recordings).
Electrophysiology: Ussing chamber studies
Staff
Lab technician Mohamed A. Ahmed, Post.doc. Morten B. Engelund, Ph.d.-student. Rikke Zachar
Courses
- Bachelor module 5 for Medical students – Electrophysiology of the heart
- SU502 for science student – General physiology and muscle cell physiology
- SU503 for science student – General physiology and muscle cell physiology
- SU503 for science students - Methods and measurements in pharmacology and Mechanisms of drug actions
- SU516 for farmaceutical students – General biology and Mechanisms of drugs actions (Per Svenningsen)
Selected publications
Svenningsen P, Andersen H, Nielsen LH, Jensen BL. Urinary serine proteases and activation of ENaC in kidney--implications for physiological renal salt handling and hypertensive disorders with albuminuria. Pflugers Arch. 2015 Mar;467(3):531-42. doi: 10.1007/s00424-014-1661-5. Epub 2014 Dec 9. PubMed PMID: 25482671.
Zachar RM, Skjødt K, Marcussen N, Walter S, Toft A, Nielsen MR, Jensen BL, Svenningsen P. The epithelial sodium channel γ-subunit is processed proteolytically in human kidney. J Am Soc Nephrol. 2015 Jan;26(1):95-106. PubMed PMID: 25060057
TMEM16A is a Ca(2+) -activated Cl(-) channel expressed in the renal collecting duct.
Svenningsen P, Nielsen MR, Marcussen N, Walter S, Jensen BL. Acta Physiol (Oxf). 2014 Oct;212(2):166-74. doi: 10.1111/apha.12323. Epub 2014 Jun 26. PMID: 24913262
Plasmin in nephrotic urine activates the epithelial sodium channel.
Svenningsen P, Bistrup C, Friis UG, Bertog M, Haerteis S, Krueger B, Stubbe J, Jensen ON, Thiesson HC, Uhrenholt TR, Jespersen B, Jensen BL, Korbmacher C, Skøtt O.
J Am Soc Nephrol. 2009 Feb;20(2):299-310. doi: 10.1681/ASN.2008040364. Epub 2008 Dec 10. PMID: 19073825
