In vivo methods (mice/rats)
- Cardiovascular disease models (myocardial infarction, atherosclerosis, aortic aneurysm, vascular injury)
- Renal disease
- Adeno-associated virus (AAV)-mediated gene delivery
- Metabolic balance studies
- Blood pressure measurements
- Microsurgery
- Ultrasound/echocardiography
In vivo methods (human)
- Insulin clamps
- Microdialysis
- Tissue biopsies
- Arterial and venous catheterization
- Ultrasound
- Metabolic studies
- Interventional studies (acute and long-term)
- Exercise interventions
Ex vivo methods
- Microdissection
- Wire and pressure myography
- Microperfusion
- Human artery biobank
- Human kidney biobank
- Kidney tubule preparations
In vitro methods
- Cell culture
- Cell transfection
- Functional assays
- Electrophysiology
Molecular Biology and Biochemistry
- Plasmid cloning techniques
- PCR/qPCR/ddPCR
- Western blotting
- Immunohistochemistry/-fluorescence
- ELISA, RIA
- HPLC
- Zymography
- Histology
- Bioluminescence
- Hormone assays (renin, aldosterone, ANGII)
- Immunoprecipitation
- cell-surface biotinylation
Imaging
- Real time NO and ROS measurements
- Fluorescent imaging
- Morphometry
- Confocal laser scanning microscopy