TARGETED PROTEOMICS
Principal investigator
Martin Overgaard, Biochemist, Associate Professor, PhD,
Martin.Overgaard@rsyd.dk; https://portal.findresearcher.sdu.dk/da/persons/movergaard
Background
Biomarker development hinges on sensitive and specific high-throughput methods for protein quantitation in cohort/routine scale.Research aim
We aim to develop MRM-MS-based targeted proteomics technology for quantitation of proteins and posttranslationally modified proteins (PTM) markers in clinical research and routine.Project examples
- First trimester biomarkers for prediction of gestational diabetes mellitus in obese and non-obese women
- Development of Clinical Applications for Evosep One (Evosep Biosystems, industrial post-doc project)
- A novel targeted multiplex mass spectrometry method for quantitation of abundant matrix and cellular proteins in formalin-fixed paraffin-embedded arterial tissue
Available cohorts and infrastructures
- Opentrons liquid handling robot
- TSQ Altis – triple quadrupole mass spectrometer (Thermo)
- Evosep One integrated HPLC (Evsosep Biosystems)
Collaborators
- Evosep Biosystems, Odense
- Yaseelan Palarasah, Assoc. Prof., IMM, SDU
- Jan Frystyk, Professor, Head of Research, Dept. of Endocrinology, OUH
- Kurt Højlund, Professor, Head of Research, Steno Diabetes Center, OUH
- Dorte Møller Jensen, Professor, Steno Diabetes Center, OUH
- Lars Melholt Rasmussen, Professor, Clin. Biochemistry, OUH
- Hans Chr. Beck, Assoc. Prof., Clin. Biochemistry, OUH
Publication examples
Steffensen LB, Stubbe J, Lindholt JS, Beck HC, Overgaard M, Bloksgaard M, Genovese F, Holm Nielsen S, Tha MLT, Bang-Moeller SK, Hong Lin MKT, Larsen JH, Hansen DR, Jones GT, Bown MJ, Karsdal MA, Rasmussen LM. Basement membrane collagen IV deficiency promotes abdominal aortic aneurysm formation. Sci Rep. (2021) 18;11(1):12903
Ravnsborg T, Svaneklink S, Andersen LLT, Larsen MR, Jensen DM, Overgaard M. First-trimester proteomic profiling identifies novel predictors of gestational diabetes mellitus. PLoS One (2019) 27;14(3)
Ravnsborg T, Andersen LL, Trabjerg ND, Rasmussen LM, Jensen DM, Overgaard M. First-trimester multimarker prediction of gestational diabetes mellitus using targeted mass spectrometry. Diabetologia. (2016) 59(5):970-9
Overgaard M, Cangemi C, Jensen ML, Argraves WS, Rasmussen LM. Total and isoform-specific quantitative assessment of circulating fibulin-1 using selected reaction monitoring MS and time-resolved immunofluorometry. Proteomics Clin Appl. (2015) 9(7-8):767-75